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KMID : 0350519930460010255
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Journal of Catholic Medical College
1993 Volume.46 No. 1 p.255 ~ p.265
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The Cellular Composition Including Lymphocyte Subsets in Human Colostrum and Mature Milk from Mothers of Preterm and Full Term Infancy
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Abstract
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This study was performed to investigate the cellular composition including lymphocyte subsets in preterm breast miik and to compare it with that in the full term breast milk.
The cellular composition of colostrum(within 72 hours postpartum) and early mature milk(5th-7th day postpartum) form 40 mothers delivering prematurely(mean gestational age: 33.9 weeks) and 40 mothers delivering at term(mean getational age: 39.7 weeks)
was examined. Lymphocyte subsets including B cells, T cells, T4 and T8 cells were measured by alkaline phosphatase anti-alkalinne phosphatase staining using wpecific monoclonal antibodies.
@ES The results were as follows ;
@EN 1. The mean total cell count in preterm colostrum(23,162.5±13,334.0μl)and milk(18.900.0±8.259.9μl) was significantly higher than in full term colostrum(13.462.5±4,971.8.μl) and milk (3,450.0±4,278.6/μl), (P<0.005).
2. Counts for macrophages, neutrophils and lymphocytes were significantly higher in preterm colostrum(15..588±9,382.1 /μl, 4,484.4±1,042.3 /μl) and milk(12,114.2±6,553.9 /μl, 4,479.3±1,489.9 /μl) as compared to those of full term
colostrum(8,679.0±3,3190 /μl, 2,2827.6±1,292.9 /μl, 1,682.5±1,077.0 /μl) and milk(5,264.5±2,717.5 /μl, 1,977.3±1,108.1 /μl, 988.2±523.9 /μl), (P<0.05).
3. Lymphocytes from preterm colostrum were 15.6±6.5% CD19+(B cell) and 80.2±6.9% CD3+(T cell), whereas lymphocytes from full term colostrum were 16.9±7.6% CD19+ and 79.7±5.6% CD3+B and Tcells in mature milk constituted 14.5±7.0% and
79.2±7.9% of
total lymphocytes in preterm group and 16.5±7.0% and 79.8±5.1% in full term group. The relative percentage distribution of B cell and T cell showed no significant difference between the two groups.
4. Lymphocte subsets from preterm colostrum were 43.8±10.8% CD4+(T4 CELL) AND 26.0±6.8% CD8+(T8 cell), whereas lymphocyte subsets from full term colostrum were 46.2±5.6% CD4+5.6% and 27.5±5.0% CD8+. T4 cells in mature milk constituted
38.4±9.1%
and 24.0±91.% of total lymphocytes in preterm group and 40.1±9.7% and 25.7±7.1% in full term group. The T cell subset distribution showed no significant difference between the two groups.
5. The T4/T8 ratios in preterm colostrum and milk were 1.75±0.43 and 1.75±0.57, whereas those in full term group were 1.74±0.34 and 1.63±0.46.
In conclusion, the higher concentration of immunocompetent cells in preterm breast milk may be a natural protective mechanism to endow the immunocompromised preterm baby with extrinsic immunologic support.
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